Focal adhesions are involved in simulated-microgravity-induced basilar and femoral arterial remodelling in rats

• DOI: 10.1139/cjpp-2017-0665
• Publication Date: 2018-03-11T22:34:24Z
• Authors: Min Jiang; Qiang Lyu; Yun-Gang Bai; Huan Liu; Jing Yang; Jiu-Hua Cheng; Ming Zheng; Jin Ma
• Keywords: Male; Focal Adhesions; 0303 health sciences; Myocytes, Smooth Muscle; Cerebral Arteries; Vascular Remodeling; Adaptation, Physiological; Femoral Artery; Rats, Sprague-Dawley; 03 medical and health sciences; src-Family Kinases; Hindlimb Suspension; Basilar Artery; Focal Adhesion Protein-Tyrosine Kinases; Animals; Collagen; Phosphorylation; Phosphotyrosine; Weightlessness Simulation

Recent studies have suggested that microgravity-induced arterial remodelling contributes to post-flight orthostatic intolerance and multiple mechanisms are involved in remodelling. However, the initial mechanism by which haemodynamic changes induce is unknown. Focal adhesions (FAs) dynamic protein complexes mechanotransduction properties. This study aimed investigate role of FAs simulated-microgravity-induced basilar femoral A 4-week hindlimb-unweighted (HU) rat model was used simulate effects microgravity, daily 1-hour intermittent artificial gravity (IAG) prevent After HU, wall thickness, volume smooth muscle cells (SMCs) collagen content were increased artery but decreased (P < 0.05). Additionally, expression p-FAK Y397 p-Src Y418 reduced SMCs arteries, respectively, HU The number Furthermore, IAG prevented HU-induced differential structural adaptations arteries. These results suggest may act as mechanosensors initiating intracellular signal transduction response altered mechanical stress induced microgravity.