Interspecific chromosomal rearrangements in monosomic addition lines of Allium

• DOI: 10.1139/g01-062
• Publication Date: 2011-04-24T14:08:22Z
• Authors: L Barthes; A Ricroch
• Keywords: Interspecific hybrids; Chromosome pairing

Monosomic alien addition lines (MAALs) are useful for assigning linkage groups to chromosomes. We examined whether the chromosomal rearrangements following introduction of a single onion (Allium cepa) chromosome into Allium fistulosum genome were produced by homeologous crossing over or nonreciprocal conversion event. Among monosomic available, 17 studied fluorescent genomic in situ hybridisation, using total A. cepa DNA as probe and competitor. In this way, such translocations between identified terminal regions consisting tandem repeats. Homeologous two closely related genomes occurred 4 lines, suggesting that events not rare. On basis detailed molecular cytogenetic characterisation, we true chromosomes 3, 4, 5, 7, 8 can reliably be used genetic studies.Key words: chromatin transfer, GISH, MAALs, Allium.