Identification by genome scanning approach (GSA) of a microsatellite tightly associated with the apple scab resistance geneVm

• DOI: 10.1139/g05-036
• Publication Date: 2005-08-11T14:17:00Z
• Authors: A Patocchi; M Walser; S Tartarini; G A.L Broggini; F Gennari; S Sansavini; C Gessler
• Keywords: Malus; Genetic linkage; Apple scab

For all known major apple scab resistance genes except Vr, molecular markers have been published. However, the precise position of some these genes, in genome, remains to be identified. Knowledge about relative is necessary preliminarily evaluate probability success their pyramidization. Pyramidization different into same genotype a reliable way create cultivars with durable resistance. Applying genome scanning approach (GSA), we identified linkage group gene Vm, derived from Malus micromalus, and found new marker tightly associated gene. The simple sequence repeat Hi07h02, previously mapped on 17, cosegregates Vm (no recombinants 95 plants tested). already published sequence-characterized amplified region OPB12 687 was linked at 5 cM and, therefore, this also maps 17 apple. This first report discovery 17. advantages using GSA for identification qualitative traits are discussed.Key words: Malus, Venturia inaequalis, mapping, repeat.