Promotion of Crystal Nucleation of Protein by Semi-Solid Agarose Gel

• DOI: 10.1143/apex.2.125501
• Publication Date: 2009-11-20T02:20:43Z
• Authors: Kana Tanabe; Mika Hirose; Ryota Murai; Shigeru Sugiyama; Noriko Shimizu; Mihoko Maruyama; Yoshinori Takahashi; Hiroaki Adachi; Kazufumi Takano; Satoshi Murakami; Yusuke Mori; Eiichi Mizohata; Tsuyoshi Inoue; Hiroyoshi Matsumura
• Keywords: Agarose; Protein crystallization; Crystal (programming language)

We investigate the nucleation rate of protein crystals in presence agarose gel at concentrations between 0.0 and 2.0% (w/v). It was found that promoted number up to a concentration 0.4% (w/v), 0.4 0.8% (w/v) decreased rate. However, increased again by more than 1.0% demonstrating high strength semi-solid gel. propose crystallization method using is powerful for screening conditions.