Bioelectrochemical Microfluidic Platform for Cultivation and Integrated Characterization of Exoelectrogenic Biofilms

• DOI: 10.1149/ma2020-01472692mtgabs
• Publication Date: 2020-05-12T19:06:49Z
• Authors: David Rehnlund; Edina Klein; Miriam Edel; Janek Weiler; Guiyeoul Lim; Katrin Sturm-Richter; Johannes Gescher
• Keywords: Shewanella oneidensis; Shewanella; Shewanella putrefaciens; Biofabrication

Microbial cultivation with the aid of microfluidic flow chambers has a great potential to form biofilms on an easy handle laboratory scale. Our platform modular design offers versatility and precision in terms simulating different environments study multiple growth conditions 1 . Thus, long-term can easily be obtained possibility integrated on-line optical analysis. In this paper we will present our recent development bioelectrochemical that allows characterization exoelectrogenic under anoxic conditions. Electroactive cultivated characterized system focusing electroactive microorganisms such as Shewanella oneidensis The conductive intracellular protein chain these microbes enables extracellular electron transfer (EET) from cytoplasm through cell membranes insoluble acceptor, anode 2 Different deletion strains were could examined compared each other by chronoamperometry, electrochemical impedance spectroscopy, cyclic voltammetry confocal laser scanning microscopy (CLSM). Genetically engineered focus probing key genes affect biofilm forming properties 3 In-situ fluorescence studies formation interaction also presented based platform. We ongoing research artificial integrate electrodeposited nanostructures biocompatible hydrogel matrix. summary, using sink studied technology. Examples well future outlooks further presented. References 1. Hansen, S. H. et al. Machine-assisted analysis biofilms. Sci. Rep. 9 , 8933 (2019). 2. Richter, K., Schicklberger, M. & Gescher, J. Dissimilatory Reduction Extracellular Electron Acceptors Anaerobic Respiration. Appl. Environ. Microbiol. 913–921 (2012). doi:10.1128/AEM.06803-11 3. Arinda, T. Addition Riboflavin-Coupled Magnetic Beads Increases Current Production Bioelectrochemical Systems via Increased Formation Anode-Biofilms. Front. 10 1–8