Lysophosphatidic acids induce proliferation of cultured vascular smooth muscle cells from rat aorta

• DOI: 10.1152/ajpcell.1994.267.1.c204
• Publication Date: 2017-12-24T17:59:10Z
• Authors: A. Tokumura; M. Iimori; Y. Nishioka; M. Kitahara; M. Sakashita; S. Tanaka
• Keywords: Lysophosphatidylcholine

Lysophosphatidic acids (LPA) with a C18 fatty acyl group accelerated thymidine incorporation into cultured rat aortic vascular smooth muscle cells and stimulated their cell division. LPA acted synergistically epidermal growth factor fibroblast but additively platelet-derived factor. The stimulatory actions of were suggested to be rather specific from the following findings: 1) stimulation DNA synthesis increased an increase in moiety; 2) lysophosphatidylcholine, neutral lysophospholipid, had no mitogenic action was cytotoxic at high concentrations; 3) induced rapid external Ca(2+)-independent intracellular Ca2+ concentration ([Ca2+]i) single fura 2-loaded that resembled receptor-mediated increases [Ca2+]i triggered by different agonists, whereas lysophosphatidylcholine provoked slow sustained Ca(2+)-dependent manner. These results are discussed relation possible pathophysiological role LPA.