Nitric oxide is necessary for a switch from stationary to locomoting phenotype in epithelial cells

• DOI: 10.1152/ajpcell.1996.270.3.c794
• Publication Date: 2017-12-24T19:04:07Z
• Authors: E. Noiri; T. Peresleni; N. Srivastava; P. Weber; W. F. Bahou; N. Peunova; M. S. Goligorsky
• Keywords: 0301 basic medicine; Base Sequence; Histocytochemistry; Movement; Molecular Sequence Data; Penicillamine; Epithelial Cells; Oligonucleotides, Antisense; Arginine; Nitric Oxide; Epithelium; Cell Line; 03 medical and health sciences; Kidney Tubules; NG-Nitroarginine Methyl Ester; Phenotype; Microscopy, Fluorescence; Chlorocebus aethiops; Animals; Nitric Oxide Synthase; Cyclic GMP; Dihydrolipoamide Dehydrogenase

The restitution of epithelial integrity is accomplished in part by cell migration. Studying this process, we have found that nitric oxide (NO) release migrating BSC-1 cells displayed a biphasic response to the inflicted wounds; an initial transient NO followed delayed sustained elevation. Whereas constitutive endothelial synthase (NOS) did not show any spatial or temporal changes associated with wounding, inducible NOS became expressed 3 h after wounding and showed higher abundance at edges wounds. L-Arginine (L-Arg) donor, S-nitroso-N-acetyl-DL-penicillamine, exerted motogenic effect cells. Inhibition NG-nitro-L-arginine methyl ester (L-NAME) selective knockout antisense oligodeoxynucleotides reduced rate spontaneous epidermal growth factor (EGF)-induced Migrating polarized expression NOS, suggesting head-to-rear gradient. Several factors (EGF, insulin-like I, hepatocyte factor, fibroblast factor) were for cells, but was abrogated pretreatment L-NAME. We conclude endogenous production prerequisite A vectorial may be essential spatially temporally coordinated reciprocal phenomena occur leading trailing edge locomoting Although exact mode action remains uncertain, it conceivable serves as cellular switch from stationary phenotype.