Intra- and extracellular measurement of reactive oxygen species produced during heat stress in diaphragm muscle

• DOI: 10.1152/ajpcell.2000.279.4.c1058
• Publication Date: 2017-12-24T23:12:23Z
• Authors: Li Zuo; Fievos L. Christofi; Valerie P. Wright; Cynthia Yu Liu; A. John Merola; Lawrence J. Berliner; Thomas L. Clanton
• Keywords: Cell Nucleus; Intracellular Fluid; Male; 0301 basic medicine; Cytoplasm; Superoxide Dismutase; Diaphragm; Cytochrome c Group; Catalase; Heat Stress Disorders; Antioxidants; Fluorescence; Respiratory Muscles; Rats; Rats, Sprague-Dawley; 03 medical and health sciences; Ethidium; 1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; Animals; Extracellular Space; Reactive Oxygen Species; Muscle Contraction

Skeletal muscles are exposed to increased temperatures during intense exercise, particularly in high environmental temperatures. We hypothesized that heat may directly stimulate the reactive oxygen species (ROS) formation diaphragm (one kind of skeletal muscle) and thus potentially play a role contractile metabolic activity. Laser scan confocal microscopy was used study conversion hydroethidine (a probe for intracellular ROS) ethidium (ET) mouse diaphragm. During 30-min period, (42 degrees C) ET fluorescence by 24 +/- 4%, whereas control (37 C), decreased 8 1% compared with baseline (P < 0.001). The superoxide scavenger Tiron (10 mM) abolished rise fluorescence, extracellular dismutase (SOD; 5,000 U/ml) had no significant effect. Reduction oxidized cytochrome c detect ROS rat After 45 min, 53 7 nmol c. g dry wt(-1). ml(-1) were reduced 22 13 nmol. g(-1). controls SOD reduction levels. results suggest stress stimulates production, which contribute physiological responses severe exercise or pathology shock.