Differential regulation of Ca2+ sparks and Ca2+ waves by UTP in rat cerebral artery smooth muscle cells

• DOI: 10.1152/ajpcell.2000.279.5.c1528
• Publication Date: 2017-12-24T23:01:22Z
• Authors: Jonathan H. Jaggar; Mark T. Nelson
• Keywords: Cyclopiazonic acid

Uridine 5′-triphosphate (UTP), a potent vasoconstrictor that activates phospholipase C, shifted Ca 2+ signaling from sparks to waves in the smooth muscle cells of rat cerebral arteries. UTP decreased frequency and transient -activated K + (K ) currents increased waves. The UTP-induced reduction spark did not reflect decrease global cytoplasmic , influx through voltage-dependent channels (VDCC), or load sarcoplasmic reticulum (SR), since was elevated, blocking VDCC prevent effect, SR decrease. However, protein kinase C (PKC) with bisindolylmaleimide I currents. effectiveness caffeine, which increases sensitivity ryanodine-sensitive release (RyR) channels, activate This work supports concept vasoconstrictors shift modalities concerted actions PKC on RyR cause sparks, inositol trisphosphate (IP 3 IP receptors generate