Two-way arginine transport in human endothelial cells: TNF-α stimulation is restricted to system y+

• DOI: 10.1152/ajpcell.2002.282.1.c134
• Publication Date: 2017-12-24T20:28:11Z
• Authors: Roberto Sala; Bianca Maria Rotoli; Emanuela Colla; Rossana Visigalli; Alessandro Parolari; Ovidio Bussolati; Gian C. Gazzola; Valeria Dall'Asta
• Keywords: Lipopolysaccharides; 0301 basic medicine; DNA Primer; Amino Acid Transport Systems; Nitrite; Nitric Oxide Synthase Type II; Gene Expression; Antineoplastic Agent; SLC7A gene; Cationic amino acid transporters; Lipopolysaccharide; Nitric oxide; SLC7A genes; System y; +; L;; Enzyme Inhibitor; Enzyme Inhibitors; Membrane Protein; Cells, Cultured; Cultured; Fusion Regulatory Protein 1, Light Chains; lipopolysaccharide; Amino Acid Transport System y+L; system y(+) L; cationic amino acid transporters; SLC7A genes; lipopolysaccharide; nitric oxide; cationic amino acid transporter; Ethylmaleimide; system y(+) L; Human; Light Chain; Amino Acid Transport System y+; Cells; 610; Antineoplastic Agents; Arginine; Nitric Oxide; 03 medical and health sciences; nitric oxide; Vascular; 616; Humans; Endothelium; Basic; Cationic Amino Acid Transporter 2; Cationic Amino Acid Transporter 1; DNA Primers; Fusion Regulatory Protein 1; Tumor Necrosis Factor-alpha; Umbilical Vein; Membrane Proteins; Biological Transport; Amino Acid Transport Systems, Basic; Endothelium, Vascular; Nitric Oxide Synthase; Carrier Protein; Carrier Proteins

Human umbilical vein endothelial cells transport arginine through two Na + -independent systems. System y L is insensitive to N-ethylmaleimide (NEM), inhibited byl-leucine in the presence of , and referable expression SLC7A6/y LAT2, SLC7A7/y LAT1, SLC3A2/4F2hc. SLC7A1/CAT1 SLC7A2/CAT2B. Tumor necrosis factor-α (TNF-α) bacterial lipopolysaccharide induce a transient stimulation influx efflux system . Increased SLC7A2/CAT2B detectable from 3 h treatment, while SLC7A1 at later times incubation. activity remain unaltered. Nitric oxide synthase type 2 mRNA not detected absence or TNF-α, latter condition lowers nitric protein level. Nitrite accumulation comparable cytokine-treated control up 48 treatment. It concluded that modulation by TNF-α occurs exclusively changes CAT2B CAT1 dissociated production.