Depletion of mitochondrial DNA alters glucose metabolism in SK-Hep1 cells

• DOI: 10.1152/ajpendo.2001.280.6.e1007
• Publication Date: 2017-12-22T12:59:46Z
• Authors: Kyu-Sang Park; Kyung-Jay Nam; Jun-Woo Kim; Youn-Bok Lee; Chang-Yeop Han; June-Key Jeong; Hong-Kyu Lee; Youngmi Kim Pak
• Keywords: Ethidium bromide; Carbohydrate Metabolism

Maternally inherited mitochondrial DNA (mtDNA) has been suggested to be a genetic factor for diabetes. Reports have shown decrease of mtDNA content in tissues diabetic patients. We investigated the effects depletion on glucose metabolism by use ρ 0 SK-Hep1 human hepatoma cells, whose was depleted long-term exposure ethidium bromide. The cells failed hyperpolarize membrane potential response stimulation. Intracellular ATP content, glucose-stimulated production, uptake, steady-state mRNA and protein levels transporters, cellular activities glucose-metabolizing enzymes were decreased compared with parental + cells. Our results suggest that quantitative reduction may suppress expression nuclear DNA-encoded transporters metabolism. Thus this lead status, such as production utilization.