Antioxidative properties of paraoxonase 2 in intestinal epithelial cells

• DOI: 10.1152/ajpgi.00039.2012
• Publication Date: 2012-06-30T00:14:06Z
• Authors: Louis-Philippe Pr...; Valérie Marcil; Thierry Ntimbane; Rame Taha; Jean-Claude Lavoie; Edgard Delvin; Ernest G. Seidman; Jean-François Bea...; Emile Levy
• Keywords: Malondialdehyde; Paraoxonase; Monocyte

Paraoxonase (PON) family members seem central to a wide variety of human illnesses, but appreciation their antioxidative function in the gastrointestinal tract is its infancy. The major objective present work highlight role ubiquitously expressed PON2 small intestine. With use pLKO lentiviral vector containing short hairpin RNA (shRNA) lentivirus, expression was knocked down intestinal Caco-2/15 cells, where status, lipid peroxidation, and degree inflammation were evaluated. As consequence inactivation epithelial we observed 1) imbalanced primary secondary responses, characterized by increased superoxide dismutases decreased catalase, 2) high concentrations H(2)O(2) malondialdehyde, along with low glutathione-to-glutathione disulfide ratio, 3) upregulation TNF-α, IL-6, monocyte chemoattractant protein-1 gene after induction oxidative stress, 4) raised level activation transcription factor NF-κB, which likely implicated exacerbation inflammatory activation. These results suggest that involved anti-inflammatory response cells.