Spontaneous Ileitis and Post-Surgical Murine Models of Enteric Hyperoxaluria

• DOI: 10.1152/ajpgi.00043.2025
• Publication Date: 2025-04-16T05:51:31Z
• Authors: Karim Jaber; Nadim Zaidan; Melody Ho; Xiaozhong Xiong; Rashmi Mishra; Ambika Nair; Arnav Mishra; Yi Chu; Mohamad Mokadem; Lama Nazzal
• Keywords: Ileitis; Jejunoileal bypass

Enteric hyperoxaluria, a risk factor for kidney stone disease, often arises from malabsorptive bariatric surgeries or inflammatory bowel diseases. Current murine models studying this condition are limited, necessitating new approaches. This study aims to establish two novel and distinct mouse investigate enteric hyperoxaluria: one simulating Roux-en-Y gastric bypass surgery the other Crohn’s ileitis. In first model, diet-induced obese C57BL/6J male mice underwent either sham surgery, followed by three weeks on high-fat, oxalate-enriched diet. second SAMP1/YitFc AKR were gradually introduced high-fat diets, later supplemented with oxalate while reducing fat content. Samples of urine, blood, feces collected assess oxalate, creatinine, fecal lipid profiles. Results showed hyperoxaluria increased stool content, indicating malabsorption, in both SAMP1 compared controls. Kidney injury was also observed. These findings confirm successful establishment models, highlighting role dietary intestinal inflammation, malabsorption disease progression. provide valuable tools exploring cellular molecular mechanisms may inform future therapeutic strategies.