Enteroendocrine cells and 5-HT availability are altered in mucosa of guinea pigs with TNBS ileitis

• DOI: 10.1152/ajpgi.00090.2004
• Publication Date: 2004-07-02T00:39:14Z
• Authors: Jennifer R. O'Hara; Winnie Ho; David R. Linden; Gary M. Mawe; Keith A. Sharkey
• Keywords: Enteroendocrine cell; Ileitis; Enterochromaffin cell; Intestinal mucosa

Enteroendocrine cells act as sensory transducers, releasing 5-HT and numerous peptides that are involved in regulating motility, secretion, gut sensation. The action of mucosal is terminated by a reuptake transporter (SERT). In this study, we examined the hypothesis ileitis leads to changes enteroendocrine cell populations availability. Ileitis was induced guinea pigs intraluminal injection 2,4,6-trinitrobenzenesulfonic acid experiments were conducted 3, 7, 14 days after treatment. number somatostatin, neurotensin, 5-HT-immunoreactive increased at 3 7 ileitis, respectively, whereas no significant numbers cholecystokinin, glucagon-like peptide-2, glucose-dependent insulinotropic peptide, peptide YY-immunoreactive observed. Chemical stimulation inflamed mucosa with sodium deoxycholic significantly release compared basal release. Mechanical potentiated effect chemical stimuli day 7. Epithelial SERT immunoreactivity reduced during time course inflammation. Thus availability could contribute altered motility secretion associated intestinal inflammation disrupting signaling enteric nerves peristaltic secretory reflexes.