Adult zebrafish intestine resection: a novel model of short bowel syndrome, adaptation, and intestinal stem cell regeneration

• DOI: 10.1152/ajpgi.00311.2014
• Publication Date: 2015-06-19T03:55:39Z
• Authors: K. A. Schall; K. A. Holoyda; C. N. Grant; D. E. Levin; E. R. Torres; A. Maxwell; H. A. Pollack; R. A. Moats; M. R. Frey; A. Darehzereshki; D. Al Alam; C. Lien; T. C. Grikscheit
• Keywords: Short Bowel Syndrome; Jejunum; Intestinal epithelium

Loss of significant intestinal length from congenital anomaly or disease may lead to short bowel syndrome (SBS); failure be partially offset by a gain in epithelial surface area, termed adaptation. Current vivo models SBS are costly and technically challenging. Operative times survival rates have slowed extension transgenic models. We created new reproducible model zebrafish, tractable vertebrate model, facilitate investigation the mechanisms Proximal diversion at segment 1 (S1, equivalent jejunum) was performed adult male zebrafish. fish emptied distal contents via stoma as human disease. After 2 wk, S1 dilated compared with controls villus ridges had increased complexity, contributing greater perimeter. The number intervillus pockets, stem cell zone zebrafish contained higher bromodeoxyuridine (BrdU)-labeled cells after wk SBS. Egf receptor subset its ligands, also drivers adaptation, were upregulated fish. Igf has been reported driver adaptation other animal models, exposed pharmacological inhibitor failed demonstrate signs such inner perimeter BrdU incorporation. describe feasible faster less expensive tool investigate plasticity well that drive