Putrescine does not support the migration and growth of IEC-6 cells

• DOI: 10.1152/ajpgi.2000.278.1.g49
• Publication Date: 2017-12-24T10:19:07Z
• Authors: Qing Yuan; Mary Jane Viar; Ramesh M. Ray; Leonard R. Johnson
• Keywords: 0301 basic medicine; Adenosylmethionine Decarboxylase; Eflornithine; Mitoguazone; Intracellular Membranes; Ornithine Decarboxylase Inhibitors; Actins; Cell Line; Rats; Drug Combinations; 03 medical and health sciences; Cell Movement; Intestine, Small; Polyamines; Putrescine; Animals; Spermine; Enzyme Inhibitors; Cell Division

The migration of IEC-6 cells is inhibited when the are depleted polyamines by inhibiting ornithine decarboxylase with alpha-difluoromethylornithine (DFMO). Exogenous putrescine, spermidine, and spermine completely restore cell DFMO. Because interconverted during their synthesis catabolism, specific role individual in intestinal migration, as well growth, remains unclear. In this study, we used an inhibitor S-adenosylmethionine decarboxylase, diethylglyoxal bis(guanylhydrazone)(DEGBG), to block spermidine from putrescine. We found that exogenous putrescine does not growth treated DFMO plus DEGBG, whereas does. addition, normal distribution actin filaments required for which disrupted polyamine-deficient cells, could be achieved adding but along DEGBG. These results indicate itself, essential it effective because converted into and/or spermine.