Expression of multidrug resistance-associated protein 2 in small intestine from pregnant and postpartum rats

• DOI: 10.1152/ajpgi.2001.280.6.g1261
• Publication Date: 2017-12-24T15:38:26Z
• Authors: Aldo D. Mottino; Tim Hoffman; Lothar Jennes; Jingsong Cao; Mary Vore
• Keywords: Brush border; Northern blot; Enterocyte

We analyzed the expression of multidrug resistance-associated protein 2 (mrp2) in small intestine control female rats and during late pregnancy (19-20 days pregnancy) lactation (2-4, 10-14, 21 after delivery). Western blot analysis was performed on brush-border membranes prepared from different regions intestine. Expression mrp2 maximal proximal segments for all experimental groups, preserved pregnant rats, increased by 100% postpartum with respect to animals. Northern mRNA revealed a positive correlation levels. Transport S-glutathione-dinitrophenol (DNP-SG) intestinal cell lumen everted sac model. Secretion DNP-SG not altered but lactating animals lactation. Intestinal mRNA, protein, transport activity are suggesting that this may represent an adaptive mechanism minimize toxicity dietary xenobiotics response food consumption.