Glycyrrhetinic derivatives inhibit hyperpolarization in endothelial cells of guinea pig and rat arteries

• DOI: 10.1152/ajpheart.2002.282.1.h335
• Publication Date: 2017-12-22T20:28:10Z
• Authors: Marianne Tare; H. A. Coleman; Helena C. Parkington
• Keywords: Hyperpolarization; Carbenoxolone; Mesenteric arteries

Glycyrrhetinic acid (GA) derivatives have been used to implicate gap junctions in vasorelaxation attributed endothelium-derived hyperpolarizing factor (EDHF). The aim of this study was assess whether GA compounds affect endothelial cell hyperpolarization. Membrane potentials were recorded from dye-identified and smooth muscle cells guinea pig coronary rat mesenteric arteries. had varied effects on the resting membrane potential: depolarization, hyperpolarization, or no effect, depending artery. 18α-GA (50 μM) a small variable effect ACh-induced hyperpolarizations cells. 18β-GA (30 carbenoxolone (100 significantly reduced both Smooth action tail arteries smaller slower presence 18β-GA. Nerve-induced excitatory junction inhibited by carbenoxolone, whereas time course their decay initially increased then decreased. In conclusion, range effects. Their inhibition EDHF hyperpolarization relaxation may stem