GM-CSF enhances lung growth and causes alveolar type II epithelial cell hyperplasia in transgenic mice

• DOI: 10.1152/ajplung.1997.273.4.l715
• Publication Date: 2017-12-23T14:17:28Z
• Authors: Jacquelyn A. Huff...; Ward R. Rice; Zsuzsanna K. Zsen...; Susan E. Wert; Glenn Dranoff; Jeffrey A. Whitsett
• Keywords: Lipopolysaccharides; Mice, Knockout; Salmonella typhimurium; 0301 basic medicine; Hyperplasia; Proteolipids; Recombinant Fusion Proteins; Body Weight; Granulocyte-Macrophage Colony-Stimulating Factor; Enzyme-Linked Immunosorbent Assay; Epithelial Cells; Mice, Transgenic; Pulmonary Surfactants; Organ Size; 3. Good health; Pulmonary Alveoli; Mice; 03 medical and health sciences; Animals; Humans; Bronchoalveolar Lavage Fluid; Lung

The human surfactant protein (SP)-C gene promoter was used to direct expression of mouse granulocyte macrophage colony-stimulating factor (GM-CSF; SP-C-GM mice) in lung epithelial cells GM-CSF-replete (GM+/+) or GM-CSF null mutant (GM−/−) mice. Lung weight and volume were significantly increased mice compared with GM+/+ GM−/− control Immunohistochemical staining demonstrated marked type II cell hyperplasia, immunofluorescent labeling for proliferating nuclear antigen Abundance per three- fourfold bromodeoxyuridine isolated vitro. Type cells, alveolar macrophages, endothelial bronchiolar stained by antibodies the receptor α-subunit both gene-targeted that are also homozygous transgene. High levels transgenic size caused demonstrating an unexpected role molecule regulation proliferation differentiation.