TIMP-1, -2, -3, and -4 in idiopathic pulmonary fibrosis. A prevailing nondegradative lung microenvironment?

• DOI: 10.1152/ajplung.2000.279.3.l562
• Publication Date: 2017-12-23T19:13:42Z
• Authors: Moises Selman; Victor Ruiz; Sandra Cabrera; Lourdes Segura; Remedios Ramírez; Roberto Barrios; Annie Pardo
• Keywords: Myofibroblast; Interstitial collagenase; Gelatinase A

Fibroblast proliferation and extracellular matrix accumulation characterize idiopathic pulmonary fibrosis (IPF). We evaluated the presence of tissue inhibitor metalloproteinase (TIMP)-1, -2, -3, -4; collagenase-1, -3; gelatinases A B; membrane type 1 (MMP) in 12 IPF 6 control lungs. TIMP-1 was found interstitial macrophages TIMP-2 fibroblast foci. TIMP-3 revealed an intense staining mainly decorating elastic lamina vessels. TIMP-4 expressed lungs by epithelial plasma cells. colocalized with Ki67 fibroblasts, whereas p27 inflammatory Collagenase-1 localized alveolar cells, collagenase-2 a few neutrophils, collagenase-3 not detected. MMP-9 neutrophils subepithelial myofibroblasts. Myofibroblast expression corroborated vitro RT-PCR. MMP-2 noticed myofibroblasts, some them close to areas basement disruption, MMP macrophages. These findings suggest that there is higher TIMPs compared collagenases, supporting hypothesis nondegrading fibrillar collagen microenvironment prevailing.