Impact of diabetes mellitus on bladder uroepithelial cells

• DOI: 10.1152/ajpregu.00129.2012
• Publication Date: 2012-11-23T01:55:13Z
• Authors: Ann T. Hanna-Mitc...; Giovanni W. Ruiz; Firouz Daneshgari; Guiming Liu; Gerard Apodaca; Lori A. Birder
• Keywords: Blood Glucose; 0301 basic medicine; Time Factors; Urinary Bladder; Apoptosis; Real-Time Polymerase Chain Reaction; Mechanotransduction, Cellular; Permeability; Diabetes Mellitus, Experimental; Rats; 3. Good health; Diabetes Complications; Rats, Sprague-Dawley; Autocrine Communication; 03 medical and health sciences; Gene Expression Regulation; Microscopy, Electron, Transmission; Paracrine Communication; Microscopy, Electron, Scanning; Animals; Female; Energy Metabolism; Cell Proliferation

Diabetic bladder dysfunction (DBD), a prevalent complication of diabetes mellitus (DM), is characterized by broad spectrum symptoms including urinary urgency, frequency, and incontinence. As DBD commonly diagnosed late, it important to understand the chronic impact DM on tissues. While changes in smooth muscle innervation have been reported diabetic patients, specialized epithelial lining bladder, urothelium (UT), largely unknown. Quantitative polymerase chain reaction analysis electron microscopy were used evaluate UT gene expression cell morphology 3, 9, 20 wk following streptozotocin (STZ) induction female Sprague-Dawley rats compared with age-matched control tissue. Desquamation superficial (umbrella) cells was noted at 9 DM, indicating possible breach barrier function. One causative factor may be metabolic burden due hyperglycemia, suggested upregulation polyol pathway glucose transport genes UT. repopulation occurred phenotype different, significant receptors associated mechanosensation (transient receptor potential vanilloid subfamily member 1; TRPV1) autocrine/paracrine signaling (acetylcholine AChR-M2 -M3, purinergic P2X(2) P2X(3)). Compromised function alterations mechanosensitivity could contribute instability, hyperactivity, altered sensation modulating activity afferent nerve endings, which appose urothelium. Our results show that impacts urothelial homeostasis underlying mechanisms DBD.