Pendrin immunoreactivity in the gill epithelium of a euryhaline elasmobranch

• DOI: 10.1152/ajpregu.00178.2002
• Publication Date: 2015-03-03T20:25:56Z
• Authors: Peter M. Piermarini; Jill W. Verlander; Ines E. Royaux; David H. Evans
• Keywords: Pendrin; Euryhaline; Stingray; Apical membrane

Pendrin is an anion exchanger in the cortical collecting duct of mammalian nephron that appears to mediate apical Cl − /HCO[Formula: see text]exchange bicarbonate-secreting intercalated cells. The goals this study were determine 1) if pendrin immunoreactivity was present gills a euryhaline elasmobranch (Atlantic stingray, Dasyatis sabina), and 2) branchial influenced by environmental salinity. Immunoblots detected Atlantic stingray gills; greatest freshwater stingrays compared with acclimated seawater (seawater acclimated) marine stingrays. Using immunohistochemistry, pendrin-positive cells on both gill lamellae interlamellar regions but more restricted seawater-acclimated gills. immunolabeling apical, discrete, intense Regardless salinity, occurred region rich basolateral vacuolar-proton-ATPase, not Na + -K -ATPase-rich We suggest pendrin-like transporter may contribute text] exchange from environments.