Obese adipose tissue modulates proinflammatory responses of mouse airway epithelial cells

• DOI: 10.1152/ajpregu.00316.2020
• Publication Date: 2021-06-09T12:00:49Z
• Authors: Jennifer L. Ather; Katherine E. Van ...; Madeleine M. Mank; Leah F. Reed; Anne E. Dixon; Matthew E. Poynter
• Keywords: Stromal vascular fraction; Proinflammatory cytokine; Diet-induced obese; Adipose tissue macrophages

Although recognized as an important endocrine organ, little is known about the mechanisms through which adipose tissue can regulate inflammatory responses in distant tissues, such lung that are affected by obesity. To explore potential mechanisms, male C57BL/6J mice were provided either high-fat diet, low-fat or a diet then switched to promote weight loss. Visceral adipocytes cultured vitro generate conditioned media (CM) was used treat both primary (mouse tracheal epithelial cells; MTECs) and immortalized (mouse-transformed club MTCCs) airway cells. Adiponectin levels greatly depressed CM from obese diet-switched relative continually fed diet. MTECs with obesity secreted higher baseline of cytokines than lean mice. treated adipocyte increased their secretion these compared CM. Diet-switched modestly decreased production CM, effects recapitulated when MTCCs. Adipose stromal vascular cells expressed genes consistent M1 macrophage phenotype eosinophil abundance fraction, profile persisted despite substantial Soluble factors exert proinflammatory effect on cells, alterations attenuated diet-induced loss, could have implications for dysfunction related asthma its mitigation