Polydatin, a natural polyphenol, protects arterial smooth muscle cells against mitochondrial dysfunction and lysosomal destabilization following hemorrhagic shock

• DOI: 10.1152/ajpregu.00350.2011
• Publication Date: 2012-01-26T06:16:43Z
• Authors: Xingmin Wang; Rui Song; Hui Ning Bian; Ulf T. Brunk; Ming Zhao; Ke-seng Zhao
• Keywords: Membrane permeability

The main objective of this study was to investigate the activity polydatin on mitochondrial dysfunction and lysosomal stability arteriolar smooth muscle cells (ASMCs) in severe shock. experimental animals (rats) were divided into five groups: control, hemorrhagic shock, shock + CsA, Res, PD (exposed cyclosporin A, resveratrol, or following induction respectively). calcein-Co(2+) technique revealed opening ASMC permeability transition pores (mPTP) after with resulting swelling, decreased membrane potential (ΔΨm), reduced intracellular ATP levels. These alterations all inhibited by exposure PD, which significantly more effective than CsA Res. also preserved stability, suppressed activation K(ATP) channels, hyperpolarization, vasoresponsiveness norepinephrine that normally follows results demonstrate initiation effectively preserves integrity has a significant therapeutic effect effects may partially result from stabilization against shock-induced oxidative stress depressed relocation hydrolytic enzymes redox-active iron that, turn, induce mPTP opening.