Enhanced pelvic responses to stressors in female CRF-overexpressing mice

• DOI: 10.1152/ajpregu.00626.2006
• Publication Date: 2006-12-28T20:23:50Z
• Authors: M. Million; L. Wang; M. P. Stenzel-Poore; S. C. Coste; P. Q. Yuan; C. Lamy; J. Rivier; T. Buffington; Y. Taché
• Keywords: Chronic Stress; Urocortin

Acute stress affects gut functions through the activation of corticotropin-releasing factor (CRF) receptors. The impact acute on pelvic viscera in context chronic is not well characterized. We investigated colonic, urinary, and locomotor responses monitored as fecal pellet output (FPO), urine voiding, ambulatory activity, respectively, female male CRF-overexpressing (CRF-OE) mice, a model, their wild-type littermates (WTL). Female CRF-OE compared with WTL, had enhanced FPO to 2-min handling (150%) 60-min novel environment (155%) but displayed similar response partial restraint stress. also significantly increased number spots (7.3 ± 1.4 vs. 1.3 0.8 spots/h) lower activity (246.8 47.8 388.2 31.9 entries/h) environment. Male mice WTL both responded failed show differences between them colonic responses. higher (113%). In CRF 1 /CRF 2 receptor antagonist astressin B selective agonist mouse urocortin (injected peripherally) prevented defecation without affecting or RT-PCR showed that receptors are expressed tissues. data stress, due continuous central overdrive, renders have altered behavioral superimposed mild stressors -initiated counteracted by receptor.