Roles of Slc13a1 and Slc26a1 sulfate transporters of eel kidney in sulfate homeostasis and osmoregulation in freshwater

• DOI: 10.1152/ajpregu.00725.2004
• Publication Date: 2005-04-01T01:37:24Z
• Authors: Tsutomu Nakada; Kambiz Zandi-Nejad; Yukihiro Kurita; Hisayuki Kudo; Vadjista Broumand; Charles Y. Kwon; Adriana Mercado; David B. Mount; Shigehisa Hirose
• Keywords: Osmoregulation; Homeostasis

Sulfate is required for proper cell growth and development of all organisms. We have shown that the renal sulfate transport system has dual roles in euryhaline eel, namely, maintenance homeostasis osmoregulation body fluids. To clarify physiological transporters teleost fish, we cloned orthologs mammalian Slc13a1 (NaSi-1) Slc26a1 (Sat-1) from eel (Anguilla japonica) assessed their functional characteristics, tissue localization, regulated expression. Full-length cDNAs coding ajSlc13a1 ajSlc26a1 were isolated a freshwater kidney cDNA library. Functional expression Xenopus oocytes revealed expected characteristics; furthermore, both inhibited by mercuric chloride. Northern blot analysis, situ hybridization, immunohistochemistry demonstrated robust apical basolateral ajSlc26a1, respectively, within proximal tubule kidney. Expression was dramatically reduced after transfer eels to seawater; circulating concentration turn markedly elevated compared with seawater conditions (19 mM vs. 1 mM). The reabsorption via may thus contribute eels, regulation concentration.