Evidence that actomyosin cross bridges contribute to “passive” tension in detrusor smooth muscle

• DOI: 10.1152/ajprenal.00635.2009
• Publication Date: 2010-04-08T00:55:22Z
• Authors: Paul H. Ratz; John E. Speich
• Keywords: Myosin Type II; 0301 basic medicine; Octoxynol; Detergents; Muscle, Smooth; Actomyosin; In Vitro Techniques; Myosins; Bridged Bicyclo Compounds, Heterocyclic; Heterocyclic Compounds, 4 or More Rings; Potassium Chloride; 03 medical and health sciences; Prostaglandin-Endoperoxide Synthases; Animals; Thiazolidines; Calcium; Cyclooxygenase Inhibitors; Female; Rabbits; Protein Kinase Inhibitors; Excitation Contraction Coupling; Muscle Contraction

Contraction of detrusor smooth muscle (DSM) at short muscle lengths generates a stiffness component we termed adjustable passive stiffness (APS) that is retained in tissues incubated in a Ca2+-free solution, shifts the DSM length-passive tension curve up and to the left, and is softened by muscle strain and release (strain softened). In the present study, we tested the hypothesis that APS is due to slowly cycling actomyosin cross bridges. APS and active tension produced by the stimulus, KCl, displayed similar length dependencies with identical optimum length values. The myosin II inhibitor blebbistatin relaxed active tension maintained during a KCl-induced contraction and the passive tension maintained during stress-relaxation induced by muscle stretch in a Ca2+-free solution. Passive tension was attributed to tension maintaining rather than tension developing cross bridges because tension did not recover after a rapid 10% stretch and release as it did during a KCl-induced contraction. APS generated by a KCl-induced contraction in intact tissues was preserved in tissues permeabilized with Triton X-100. Blebbistatin and the actin polymerization inhibitor latrunculin-B reduced the degree of APS generated by a KCl-induced contraction. The degree of APS generated by KCl was inhibited to a greater degree than was the peak KCl-induced tension by rhoA kinase and cyclooxygenase inhibitors. These data support the hypothesis that APS is due to slowly cycling actomyosin cross bridges and suggest that cross bridges may play a novel role in DSM that uniquely serves to ensure proper contractile function over an extreme working length range.