The Association of Myostatin on the Expression of Inflammatory Cytokines and Chemokines in Rheumatoid Arthritis Synovial Fibroblasts

• DOI: 10.1152/physiol.2024.39.s1.1020
• Publication Date: 2024-05-21T14:57:50Z
• Authors: Samudra Lansakara; Yong soo Kim; Janis J.Weis; Chathura Siriward...
• Keywords: Proinflammatory cytokine; Myostatin

Rheumatoid arthritis (RA) is a chronic autoimmune disease initiated and progressed by RA synovial fibroblast cells (RASFs) of the synovium. Inflammatory cytokines/chemokines play crucial role in development. Myostatin (MSTN) widely comprehended as significant regulator pro-inflammatory many other diseases. However, its during progression still unclear. We hypothesized that MSTN regulates inflammatory expression RASFs. The immortalized RASF (MH7A) were treated with or inhibitor (0, 10, 20 ng/mL) for 0, 24, 48 h. Then, secretion cytokines (IL-8, IL-17, TNF-α, IL-6, IL-23, IFN-γ, IFN-β) chemokines (CCL-2, CCL-20, CXCL-13, CXCL-1) measured enzyme-linked immunosorbent assay (ELISA) reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Furthermore, MH7A ng/mL IFN-γ IFN-β, CCL-2, CXCL-1 24 h to examine their effects on secretion. Data analyzed Kruskal-Wallis rank sum test, followed Dunn’s multiple comparison test. RT-qPCR, ELISA, Western blot. Results showed treatment significantly (p<0.05) increased most at highest (30-fold average) response treatment, CCL-2 (13-fold IL-23 (3-fold Similar ELISA results, upregulated mRNA was TNF-α (60-fold h, (15-fold effect inconsistent. Among six used investigate cells, (100-fold but cytokine/chemokines no response. blot results supporting results. Current demonstrated various secretion, indicating cross-stimulation between RA. This study funded NIH 5R01AR043521-29. full abstract presented American Physiology Summit 2024 meeting only available HTML format. There are additional versions content this abstract. not involved peer review process.