Gene expression following acute morphine administration

• DOI: 10.1152/physiolgenomics.2001.6.3.169
• Publication Date: 2017-12-23T23:53:49Z
• Authors: A. V. LOGUINOV; L. M. ANDERSON; G. J. CROSBY; R. Y. YUKHANANOV
• Keywords: Lumbar Spinal Cord

The long-term response to neurotropic drugs depends on drug-induced neuroplasticity and underlying changes in gene expression. However, alterations neuronal expression can be observed even following single injection. To investigate the extent of these changes, medial striatum lumbar part spinal cord was monitored by cDNA microarray injection morphine. Using robust resistant linear regression (MM-estimator) with simultaneous prediction confidence intervals, we detected differentially expressed genes. By combining results cluster analysis, have found that a morphine alters two major groups genes, for proteins involved mitochondrial respiration cytoskeleton-related proteins. RNAs were mostly downregulated both cord. These transitory prevented coadministration opioid antagonist naloxone. Data indicate analysis itself is useful describing effect well-known substances nervous system provides sufficient information propose potentially novel pathway mediating its activity.