Lectins from the Red Marine Algal SpeciesBryothamnion seaforthiiandBryothamnion triquetrumas Tools to Differentiate Human Colon Carcinoma Cells

• DOI: 10.1155/2009/862162
• Publication Date: 2009-12-10T07:07:07Z
• Authors: Vicente P. T. Pinto; Henri Debray; Danuta Dus; Edson H. Teixeira; Taianá Maia de Ol...; Victor Alves Carn...; Alrieta H. Teixeira; Gerardo C. Filho; Celso S. Nagano; Kyria S. Nascimento; Alexandre H. Sampaio; Benildo S. Cavada
• Keywords: Internalization

The carbohydrate-binding activity of the algal lectins from closely related red marine species Bryothamnion triquetrum (BTL) and seaforthii (BSL) was used to differentiate human colon carcinoma cell variants with respect their membrane glyco-receptors. These interacted cells tested in a dose-dependent manner. Moreover, fluorescence spectra both clearly differentiated as shown by FACS profiles. Furthermore, observed confocal microscopy, BTL BSL bound surface glycoproteins underwent intense internalization, which makes them possible tools targeting strategies.