Phenethyl Isothiocyanate (PEITC) Inhibits the Growth of Human Oral Squamous Carcinoma HSC-3 Cells throughG0/G1Phase Arrest and Mitochondria-Mediated Apoptotic Cell Death

• DOI: 10.1155/2012/718320
• Publication Date: 2012-07-10T21:46:02Z
• Authors: Po-Yuan Chen; Kai-Chun Lin; Jing-Pin Lin; Nou-Ying Tang; Jai-Sing Yang; Kung-Wen Lu; Jing-Gung Chung
• Keywords: Phenethyl isothiocyanate; Squamous carcinoma

Phenethyl isothiocyanate (PEITC), an effective anticancer and chemopreventive agent, has been reported to inhibit cancer cell growth through cell-cycle arrest induction of apoptotic events in various human cells models. However, whether PEITC inhibits oral squamous carcinoma HSC-3 its underlying mechanisms is still not well elucidated. In the present study, we evaluated inhibitory effects examined PEITC-modulated apoptosis. The contrast-phase flow cytometric assays were used for examining morphological changes viability, respectively. apoptosis-associated protein levels determined utilizing Western blotting after exposure PEITC. Our results indicated that effectively inhibited cells’ caused induced<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msub><mml:mrow><mml:mi>G</mml:mi></mml:mrow><mml:mrow><mml:mn>0</mml:mn></mml:mrow></mml:msub><mml:mo>/</mml:mo><mml:msub><mml:mrow><mml:mi>G</mml:mi></mml:mrow><mml:mrow><mml:mn>1</mml:mn></mml:mrow></mml:msub></mml:math>phase associated such as p53, p21, p17, CDK2 cyclin E, it triggered apoptosis promotion Bax Bid expression reduction Bcl-2, leading decrease mitochondrial membrane potential (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msub><mml:mrow><mml:mi>Δ</mml:mi><mml:mi>Ψ</mml:mi></mml:mrow><mml:mrow><mml:mi>m</mml:mi></mml:mrow></mml:msub></mml:math>), followed releases cytochrome<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>c</mml:mi></mml:math>, AIF Endo G then causing cells. These suggest could be antitumor compound therapy.