Biphasic Activation of Nuclear Factor-Kappa B in Experimental Models of Subarachnoid HemorrhageIn VivoandIn Vitro

• DOI: 10.1155/2012/786242
• Publication Date: 2012-09-23T17:02:41Z
• Authors: Wan-Chun You; Wei Li; Zong Zhuang; Yong Tang; Hu-Chen Lu; Xiang-Jun Ji; Wei Shen; Ji-Xin Shi; Meng-Liang Zhou
• Keywords: Male; L-Lactate Dehydrogenase; NF-kappa B; Electrophoretic Mobility Shift Assay; Subarachnoid Hemorrhage; Real-Time Polymerase Chain Reaction; 3. Good health; Disease Models, Animal; Mice; 03 medical and health sciences; 0302 clinical medicine; Pregnancy; Pathology; RB1-214; Animals; Female; Rabbits; Research Article

It has been proven that nuclear factor-kappa B (NF-κB) is activated as a well-known transcription factor after subarachnoid hemorrhage (SAH). However, the panoramic view of NF-κB activity after SAH remained obscure. Cultured neurons were signed into control group and six hemoglobin- (Hb-) incubated groups. One-hemorrhage rabbit SAH model was produced, and the rabbits were divided randomly into one control group and five SAH groups. NF-κB activity was detected by electrophoretic mobility shift assay (EMSA) and immunohistochemistry. Real-time polymerase chain reaction (PCR) was performed to assess the downstream genes of NF-κB. NeuN immunofluorescence and lactate dehydrogenase (LDH) quantification were used to estimate the neuron injury. Double drastically elevated NF-κB activity peaks were detected in rabbit brains and cultured neurons. The downstream gene expressions showed an accordant phase peaks. NeuN-positive cells decreased significantly in day 3 and day 10 groups. LDH leakage exhibited a significant increase in Hb-incubated groups, but no significant difference was found between the Hb incubated groups. These results suggested that biphasic increasing of NF-κB activity was induced after SAH, and the early NF-κB activity peak indicated the injury role on neurons; however, the late peak might not be involved in the deteriorated effect on neurons.