Moscatilin Inhibits Lung Cancer Cell Motility and Invasion via Suppression of Endogenous Reactive Oxygen Species

• DOI: 10.1155/2013/765894
• Publication Date: 2013-05-08T21:35:51Z
• Authors: Akkarawut Kowitda...; Pithi Chanvorachote; Boonchoo Sritularak; Varisa Pongrakhan...
• Keywords: 0303 health sciences; Lung Neoplasms; Cell Death; Models, Biological; Neoplasm Proteins; 3. Good health; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Focal Adhesion Protein-Tyrosine Kinases; Benzyl Compounds; Humans; Neoplasm Invasiveness; Pseudopodia; Drug Screening Assays, Antitumor; Reactive Oxygen Species; Research Article; Signal Transduction

Lung cancer is the leading cause of death among patients worldwide, and most them have died from metastasis. Migration invasion are prerequisite processes associated with high metastasis potential in cancers. Moscatilin, a bibenzyl derivative isolated Thai orchid Dendrobium pulchellum , has been shown to anticancer effect against numerous cell lines. However, little known regarding moscatilin on migration invasion. The present study demonstrates that nontoxic concentrations were able inhibit human nonsmall lung H23 inhibitory was an attenuation endogenous reactive oxygen species (ROS), which hydroxyl radical (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:msup><mml:mrow><mml:mtext>OH</mml:mtext></mml:mrow><mml:mrow><mml:mo>∙</mml:mo></mml:mrow></mml:msup></mml:mrow></mml:math>) identified as dominant suppression filopodia formation. Western blot analysis also revealed downregulated activated focal adhesion kinase (phosphorylated FAK, Tyr 397) ATP-dependent tyrosine Akt, Ser 473), whereas their parental counterparts not detectable changed. In conclusion, our results indicate novel molecular basis moscalitin-inhibiting motility demonstrate promising antimetastatic such agent for therapy.