LPS- and LTA-Induced Expression of IL-6 and TNF-αin Neonatal and Adult Blood: Role of MAPKs and NF-κB

• DOI: 10.1155/2014/283126
• Publication Date: 2014-10-30T17:02:31Z
• Authors: Lutz Koch; David Frommhold; Kirsten Buschmann; Navina Kuss; Johannes Poeschl; Peter Ruef
• Keywords: Lipoteichoic acid; Cord blood; IκBα

As nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) seem to be critical mediators in the inflammatory response, we studied effects of lipopolysaccharide (LPS) lipoteichoic acid (LTA) on (a) activation NF-κB MAPKs (b) expression tumor necrosis alpha (TNF-α) interleukin 6 (IL-6) with or without specific inhibitors these intracellular signal transduction pathways neonatal cord adult blood. TNF-α IL-6 concentrations showed a sharp increase supernatants whole blood after stimulation. were significantly higher, whereas tendentially lower Stimulation LPS LTA resulted decreased p38 MAPK compared Although failed induce additional ERK1/2 phosphorylation, stimulation mediated moderately increased levels activated monocytes. The addition inhibitor SB202190 production upon Furthermore, inhibition was able reduce LPS-stimulated We conclude that as well phosphorylation is crucially involved could explain differences early cytokine response between