Silver Nanoparticle Synthesis Using Monosaccharides and Their Growth Inhibitory Activity against Gram-Negative and Positive Bacteria

• DOI: 10.1155/2014/480284
• Publication Date: 2014-02-12T21:02:50Z
• Authors: Colin Pettegrew; Zheng Dong; M. Zubayed Muhi; Scott Pease; M. Abdul Mottaleb; M. Rafiq Islam
• Keywords: Monosaccharide; Bacterial growth

Using various monosaccharides as reductant, we synthesized Ag nanoparticles (NPs) in seconds employing the household microwave method described earlier. The NPs containing colloidal solution showed distinctive colors with varying <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:msub><mml:mrow><mml:mi>λ</mml:mi></mml:mrow><mml:mrow><mml:mtext>max</mml:mtext></mml:mrow></mml:msub></mml:mrow></mml:math>. sizes of formed varied significantly from 10 to 35 nm good agreement localized plasmon resonance ranged ~300 ~600 nm. antimicrobial properties these were compared Gram-negative and positive bacteria liquid culture. Gram-positive highly susceptible microbes—the additional lipopolysaccharide layer covering peptidoglycan cell wall latter somewhat lessens effect. results indicated that larger produced by glucose inhibited bacterial growth better than smallest ribose. This may be attributed higher aggregation rate for on wall. SEM analysis accumulation surface defect budding, further supporting interaction NPs. These observations suggested inhibition is mediated interfering peptidoglycan.