Reference Gene Selection for Quantitative Real-Time RT-PCR Normalization inIris. lacteavar.chinensisRoots under Cadmium, Lead, and Salt Stress Conditions

• DOI: 10.1155/2014/532713
• Publication Date: 2014-05-26T21:04:21Z
• Authors: Chun-Sun Gu; Liang-qin Liu; Chen Xu; Yan-hai Zhao; Xu-dong Zhu; Su-Zhen Huang
• Keywords: Reference Genes; Elongation factor

Quantitative real time PCR (RT-qPCR) has emerged as an accurate and sensitive method to measure the gene expression. However, obtaining reliable result depends on selection of reference genes which normalize differences among samples. In this study, we assessed expression stability seven genes, namely, ubiquitin-protein ligase UBC9 ( UBC ), tubulin alpha-5 TUBLIN eukaryotic translation initiation factor EIF-5A elongation EF1A EF1 α EF1B EF1b actin11 ACTIN histone H3 HIS in Iris. lactea var. chinensis I. ) root when plants were subjected cadmium (Cd), lead (Pb), salt stress conditions. All showed a relatively wide range threshold cycles (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:msub><mml:mrow><mml:mi>C</mml:mi></mml:mrow><mml:mrow><mml:mi>t</mml:mi></mml:mrow></mml:msub></mml:mrow></mml:math>) values different GeNorm NormFinder algorithms used assess suitable genes. The results from two software units that most stable across all tested samples, while was unsuitable internal controls. is tolerant Cd, Pb, salt. Our will benefit future research response three abiotic stresses.