Antifungal, Antileishmanial, and Cytotoxicity Activities of Various Extracts of Berberis vulgaris (Berberidaceae) and Its Active Principle Berberine

• DOI: 10.1155/2014/602436
• Publication Date: 2014-03-10T21:02:57Z
• Authors: Hossein Mahmoudvand; Seyyed Amin Ayato...; Asghar Sepahvand; Fariba Sharififar; Behrouz Ezatpour; Fatemeh Gorohi; Ebrahim Saedi Dezaki; Sareh Jahanbakhsh
• Keywords: Berberidaceae; MTT assay; Microsporum gypseum

In this study, in vitro antidermatophytic activity against Trichophyton mentagrophytes , rubrum Microsporum canis and gypseum was studied by disk diffusion test assessment of minimum inhibitory concentration (MIC) using CLSI broth macrodilution method (M38-A2). Moreover, antileishmanial cytotoxicity B. vulgaris berberine promastigotes Leishmania major tropica were evaluated colorimetric MTT assay. The findings indicated that the various extracts particularly showed high potential pathogenic dermatophytes tested with MIC values varying from 0.125 to >4 mg/mL. results revealed as well effective inhibiting L. growth a dose-dependent manner IC 50 (50% concentration) 2.1 26.6 μ g/mL. it could be observed compared exhibited more murine macrophages CC (cytotoxicity for 50% cells) 27.3 362.6 Results investigation first step search new drugs. However, further works are required evaluate exact effect these animal models volunteer human subjects.