Reexpression of Let-7g MicroRNA Inhibits the Proliferation and Migration via K-Ras/HMGA2/Snail Axis in Hepatocellular Carcinoma

• DOI: 10.1155/2014/742417
• Publication Date: 2014-03-04T16:09:28Z
• Authors: Ke-ji Chen; Ying Hou; Kui Wang; Jun Li; Yong Xia; Xiao-yu Yang; Gang Lv; Xiang-Lei Xing; Feng Shen
• Keywords: Male; Mice, Inbred BALB C; 0303 health sciences; Carcinoma, Hepatocellular; HMGA2 Protein; Liver Neoplasms; Mice, Nude; 3. Good health; Gene Expression Regulation, Neoplastic; Proto-Oncogene Proteins p21(ras); Mice; MicroRNAs; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Proto-Oncogene Proteins; Animals; Humans; Female; RNA, Neoplasm; Snail Family Transcription Factors; Research Article; Cell Proliferation; Transcription Factors

Let-7 family microRNAs have been reported to be downregulated in human hepatocellular carcinoma comparison with normal hepatic tissues. Among them, let-7g was identified as the lowest expression using real-time RT-PCR. However, mechanism by which works remains unknown. Here, our present study, we had reexpressed vitro cell lines MHCC97-H and HCCLM3 via transfection. The proliferation after reexpression of assayed MTT method; migration invasion restoration were detected wound-healing Transwell assay, respectively. We found Western-blotting that can regulate epithelial-mesenchymal transition (EMT) downregulating K-Ras HMGA2A reexpresssion. Xenografted nude mice used observe whether or not could potential therapeutic ability. In vivo , association overall prognosis, 40 paired cases analyzed situ hybridization (ISH). It inhibit proliferation, migration, significantly, low significantly associated poorer survival. Taken together, a promising agent treatment at earlier stage.