Angiogenin Expression during Early Human Placental Development; Association with Blood Vessel Formation

• DOI: 10.1155/2014/781632
• Publication Date: 2014-07-02T08:55:33Z
• Authors: Nadine Pavlov; Jean-Louis Frendo; Jean Guibourdenche; Séverine A. Degrelle; Danièle Evain-Brion; Josette Badet
• Keywords: Angiogenin; Vasculogenesis; Decidua; Syncytiotrophoblasts

The placenta is a transient organ essential for fetal development. During human placental development, chorionic villi grow in coordination with large capillary network resulting from both vasculogenesis and angiogenesis. Angiogenin one of the most potent inducers neovascularisation experimental models vivo. We others have previously mapped angiogenin expression term placenta. Here, we explored involvement early studied, by situ hybridisation and/or RT-PCR tissues primary cultured trophoblastic cells cellular distribution coimmunolabelling cell markers: CD31 (PECAM-1), vascular endothelial cadherin (VE-cadherin), growth factor receptor-2 (VEGF-R2), Tie-2, von Willebrand factor, CD34, erythropoeitin receptor (Epo-R), alpha-smooth muscle actin, CD45, cytokeratin 7, Ki-67. Extravillous villous cytotrophoblasts, isolated differentiated vitro, expressed secreted angiogenin. was detected layers, structured nascent vessels. In decidua, it glandular epithelial cells, macrophages. observed pattern compatible role blood vessel formation cross-talk between trophoblasts cells. view properties, suggest that may participate organogenesis.