Decorin-Mediated Inhibition of Human Trophoblast Cells Proliferation, Migration, and Invasion and Promotion of ApoptosisIn Vitro

• DOI: 10.1155/2015/201629
• Publication Date: 2015-08-19T17:03:02Z
• Authors: Yanfen Zou; Xiang Yu; Jing Lu; Ziyan Jiang; Qing Zuo; Mingsong Fan; Shiyun Huang; Lizhou Sun
• Keywords: Adult; 0301 basic medicine; Placenta; Apoptosis; Trophoblasts; 3. Good health; Young Adult; 03 medical and health sciences; Matrix Metalloproteinase 9; Pre-Eclampsia; Cell Movement; Pregnancy; Cell Line, Tumor; Decidua; Humans; Matrix Metalloproteinase 2; Female; Decorin; Research Article; Cell Proliferation

Preeclampsia (PE) is a unique complication of pregnancy, the pathogenesis of which has been generally accepted to be associated with the dysfunctions of extravillous trophoblast (EVT) including proliferation, apoptosis, and migration and invasion. Decorin (DCN) has been proved to be a decidua-derived TGF-binding proteoglycan, which negatively regulates proliferation, migration, and invasiveness of human extravillous trophoblast cells. In this study, we identified a higher expression level of decorin in severe PE placentas by both real-time reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC). And an inhibitory effect of decorin on proliferation, migration, and invasion and an enhanced effect on apoptosis in trophoblast cells HTR-8/SVneo and JEG-3 were validatedin vitro. Also the modulations of decorin on trophoblast cells’ metastasis and invasion functions were detected through regulating the matrix metalloproteinases (MMP2 and MMP9). Thus, we suggested that the contribution of decorin to the modulation of trophoblast cells might have implications for the pathogenesis of preeclampsia.