Quantitative Shotgun Proteomics Analysis of Rice Anther Proteins after Exposure to High Temperature

• DOI: 10.1155/2015/238704
• Publication Date: 2015-11-05T16:01:04Z
• Authors: Mijeong Kim; Hijin Kim; Wondo Lee; Yoonjung Lee; Soon-Wook Kwon; Joohyun Lee
• Keywords: Proteome; Shotgun proteomics; Shotgun; Antifreeze protein

In rice, the stage of development most sensitive to high temperature stress is flowering, and exposure at this can result in spikelet sterility, thereby leading significant yield losses. study, protein expression patterns rice anthers from Dianxi4, a tolerant Japonica variety, were compared between samples exposed those grown natural field conditions Korea. Shotgun proteomics analysis three replicate control high-temperature-treated identified 3,266 nonredundant anther proteins (false discovery rate < 0.01). We found that levels ATP synthase, cupin domain-containing proteins, pollen allergen present anthers. Comparative analyses 1,944 reproducibly expressed 139 differentially with 95 increased 44 decreased response conditions. Heat shock, DnaK family, chaperone showed highly expression, suggesting tolerance Dianxi4 achieved by stabilization cells. Trehalose synthase was also after heat treatment, possible role for trehalose preventing denaturation through desiccation.