Improvement ofIn VitroOsteogenic Potential through Differentiation of Induced Pluripotent Stem Cells from Human Exfoliated Dental Tissue towards Mesenchymal-Like Stem Cells

• DOI: 10.1155/2015/249098
• Publication Date: 2015-01-31T16:00:16Z
• Authors: Felipe Augusto An...; Roberto Dalto Fan...; Karina Griesi-Oli...; Angela May Suzuki; Gerson Shigeru Ko...; Andressa Gois Mor...; Luciane Portas Ca...; Maria Rita Passos...
• Keywords: CD90

Constraints for the application of MSCs bone reconstruction include restricted self-renewal and limited cell amounts. iPSC technology presents advantages over MSCs, providing homogeneous cellular populations with prolonged higher plasticity. However, it is unknown if osteogenic potential iPSCs differs from that depends on originating source. Here, we compared in vitro osteogenesis between stem cells human deciduous teeth (SHED) MSC-like SHED (iPS-SHED) dermal fibroblasts (iPS-FIB). iPS-SHED iPS-FIB displayed fibroblast-like morphology, downregulation pluripotency markers upregulation mesenchymal markers. Comparative analysis showed followed by SHED. CD105 expression, reported to be inversely correlated did not display this pattern, considering presented lower expression. Higher may due homogeneity and/or donor tissue epigenetic memory. Our findings strengthen rationale use bioengineering. Unveiling molecular basis behind these differences important a thorough clinical scenarios.