TrkB-Mediated Neuroprotective and Antihypoxic Properties of Brain-Derived Neurotrophic Factor

• DOI: 10.1155/2015/453901
• Publication Date: 2015-05-13T17:08:26Z
• Authors: Maria V. Vedunova; Tatiana A. Mishch...; Elena V. Mitroshina; Irina V. Mukhina
• Keywords: Hypoxia; Trk receptor

The neuroprotective and antihypoxic effects of brain-derived neurotrophic factor (BDNF) on dissociated hippocampal cultures in a hypoxia model were investigated. These experiments demonstrate that 10 minutes normobaric increased the number dead cells primary culture, whereas preventive application BDNF viable cells. Spontaneous bioelectrical calcium activity neural networks was analyzed using multielectrode arrays functional intravital imaging. results indicate affects parameters neuronal over 7-day posthypoxic period. In addition, k252a, an antagonist tropomyosin-related kinase B (TrkB), during after acute It shown protective are associated with binding to TrkB receptor. Finally, fluorescent mRNA probes used study role NF- κ B1 BDNF. Our revealed stimulates synthesis under normal conditions but not hypoxic state.