LPS from P. gingivalis Negatively Alters Gingival Cell Mitochondrial Bioenergetics

• DOI: 10.1155/2017/2697210
• Publication Date: 2017-05-16T17:00:54Z
• Authors: Kiran Napa; Andrea C. Baeder; Jeffrey E. Witt; Sarah T. Rayburn; Madison G. Miller; Blake W. Dallon; Jonathan L. Gibbs; Shalene H. Wilcox; Duane R. Winden; Jared H. Smith; Paul R. Reynolds; Benjamin T. Bikman
• Keywords: Bioenergetics

Objective . Oral inflammatory pathologies are linked to increased oxidative stress, thereby partly explaining their relevance in the etiology of systemic disorders. The purpose this work was determine degree which LPS from Porphyromonas gingivalis , primary pathogen related oral inflammation, altered gingival mitochondrial function and reactive oxygen species generation. Methods Human fibroblast (HGF-1) cells were treated with lipopolysaccharide P. gingivalis. Mitochondrial determined via high-resolution respirometry. Results LPS-treated HGF-1 had significantly higher complex IV rates respiration. However, failed translate into greater ATP production, as production paradoxically diminished treatment. Nevertheless, H 2 O elevated Conclusions elicits an increase cell mitochondria content, a subsequent (i.e., ), despite paradoxical reduction These findings provide insight nature stress pathologies.