Data from Real-time Imaging of Tumor-Cell Shedding and Trafficking in Lymphatic Channels

• DOI: 10.1158/0008-5472.c.6495627.v1
• Publication Date: 2023-03-30T21:37:37Z
• Authors: Katsuhiro Hayashi; Ping Jiang; Kensuke Yamauchi; Norio Yamamoto; Hiroyuki Tsuchiya; Katsuro Tomita; A.R. Moossa; Michael Bouvet; Robert M. Hoffman
• Keywords: Lymphatic vessel; Live cell imaging; Fluorescence-lifetime imaging microscopy

<div>Abstract<p>In the present report, we show real-time imaging of cancer cell trafficking in lymphatic vessels. Cancer cells labeled with both green fluorescent protein (GFP) nucleus and red (RFP) cytoplasm or GFP only RFP were injected into inguinal lymph node nude mice. The trafficked through vessels where they imaged via a skin flap real time at cellular level until entered axillary node. bright fluorescence microscopic capability Olympus OV100 small-animal system enabled lymphatics. Using this strategy, two different lines, one expressing other RFP, simultaneously Fluorescence readily distinguished color-coded lines their abilities to survive system. technology, also investigated role pressure on tumor-cell shedding Pressure was generated by placing 25- 250-g weights for 10 s bottom surface tumor-bearing footpad. Tumor fragments, single cells, emboli shed from footpad tumor easily Increasing increased numbers emboli. deformed emboli, increasing maximum major axis. Imaging can reveal critical steps metastasis. [Cancer Res 2007;67(17):8223–8]</p></div>