Data from miR-590-3p Promotes Ovarian Cancer Growth and Metastasis via a Novel FOXA2–Versican Pathway

• DOI: 10.1158/0008-5472.c.6510213.v1
• Publication Date: 2023-03-31T22:05:53Z
• Authors: Mohamed Salem; Jacob A. O'Brien; Stefanie Bernaudo; Heba Shawer; Gang Ye; Jelena Brkić; Asma Amleh; Barbara C. Vander...; Basel Refky; Burton B. Yang; Sergey N. Krylov; Chun Peng
• Keywords: FOXA2

<div>Abstract<p>miRNAs play important roles in gene regulation, and their dysregulation is associated with many diseases, including epithelial ovarian cancer (EOC). In this study, we determined the expression function of miR-590-3p EOC. levels were higher high-grade carcinoma when compared low-grade or tumors low malignant potential. Interestingly, plasma significantly patients EOC than subjects benign gynecologic disorders. Transient transfection mimics stable mir-590 increased cell proliferation, migration, invasion. <i>In vivo</i> studies revealed that accelerated tumor growth metastasis. Using a cDNA microarray, identified forkhead box A2 (FOXA2) versican (VCAN) as top downregulated upregulated genes by mir-590, respectively. targeted FOXA2 3′ UTR to suppress its expression. addition, knockdown knockout enhanced Overexpression decreased, whereas VCAN mRNA protein levels, which was due direct binding regulation FOXA2. Interrogation TCGA database negative relationship between tumors, high FOXA2/low positively correlated patient survival. Finally, overexpression silencing reversed effects mir-590. These findings demonstrate promotes development via novel FOXA2–VCAN pathway.</p><p><b>Significance:</b> Low FOXA2/high mediate tumor-promoting negatively correlate <i>Cancer Res; 78(15); 4175–90. ©2018 AACR</i>.</p></div>