Data from EBF1-Mediated Upregulation of Ribosome Assembly Factor PNO1 Contributes to Cancer Progression by Negatively Regulating the p53 Signaling Pathway

• DOI: 10.1158/0008-5472.c.6511649.v1
• Publication Date: 2023-03-31T21:52:02Z
• Authors: Aling Shen; Youqin Chen; Liya Liu; Yue Huang; Hongwei Chen; Fei Qi; Jiumao Lin; Zhiqing Shen; Xiangyan Wu; Meizhu Wu; Qiongyu Li; Liman Qiu; Na Yu; Thomas J. Sferra; Jun Peng
• Keywords: ribosome biogenesis; Viability assay

<div>Abstract<p>The RNA-binding protein PNO1 is critical for ribosome biogenesis, but its potential role in cancer remains unknown. In this study, online data mining, cDNA, and tissue microarrays indicated that expression was higher colorectal than noncancerous tissue, overexpression associated with worse patient survival. Gain-of-function loss-of-function studies demonstrated knockdown suppressed growth of cells <i>in vitro</i> vivo</i>, while promoted cell proliferation vitro</i>. expressing wild-type p53, enhanced p53 downstream gene p21, reduced viability; these effects were prevented by knockout attenuated the inhibitor PFT-α. Moreover, HCT116 decreased levels 18S rRNA, 40S 60S ribosomal subunits, 80S ribosome. It also global synthesis, increasing nuclear stress inhibiting MDM2-mediated ubiquitination degradation. Overexpressing EBF1 promoter activity mRNA protein, inducing apoptosis through p53/p21 pathway. tissues, correlated inversely PNO1. Data mining breast lung databases showed increased association poor survival; viability cultured cells. Taken together, findings indicate overexpressed correlates survival, exerts oncogenic effects, at least, part, altering biogenesis.</p>Significance:<p>This study identifies assembly factor as a oncogene involved tumor progression cancer.</p></div>