Data from Characterizing Macrophage Diversity in Metastasis-Bearing Lungs Reveals a Lipid-Associated Macrophage Subset

• DOI: 10.1158/0008-5472.c.6513516.v1
• Publication Date: 2023-03-31T21:20:10Z
• Authors: Danielle N. Huggins; Rebecca S. LaRue; Ying Wang; Todd P. Knutson; Yingzheng Xu; Jesse W. Williams; Kathryn L. Schwer...
• Keywords: M2 Macrophage

<div>Abstract<p>While macrophages are among the most abundant immune cell type found within primary and metastatic mammary tumors, how their complexity heterogeneity change with progression remains unknown. Here, were isolated from lungs of mice bearing orthotopic tumors for single-cell RNA sequencing (scRNA-seq). Seven distinct macrophage clusters identified, including populations exhibiting enhanced differential expression genes related to antigen presentation (<i>H2-Aa, Cd74</i>), cycle (<i>Stmn1, Cdk1</i>), interferon signaling (<i>Isg15, Ifitm3</i>). Interestingly, one cluster demonstrated a profile concordant lipid-associated (<i>Lgals3, Trem2</i>). Compared nontumor-bearing controls, number these cells per gram tissue was significantly increased in tumor-bearing mice, vast majority costaining positively alveolar marker Siglec-F. Enrichment implicated pathways lipid metabolism as well extracellular matrix remodeling immunosuppression observed. In addition, displayed reduced capacity phagocytosis. Collectively, findings highlight diversity present lesions characterize subset previously unidentified lung metastases.</p>Significance:<p>scRNA-seq metastases reveals extensive identifies novel subpopulation enriched involved metabolism, remodeling, immunosuppression.</p></div>