Inverse Prognostic Impact of Angiogenic Marker Expression in Tumor Cells versus Stromal Cells in Non–Small Cell Lung Cancer

• DOI: 10.1158/1078-0432.ccr-07-0414
• Publication Date: 2007-11-15T20:23:19Z
• Authors: Tom Donnem; Samer Al-Saad; Khalid Al-Shibli; Marit P. Delghandi; Magnus Persson; Marit N. Nilsen; Lill-Tove Busund; Roy M. Bremnes
• Keywords: Tissue microarray; Lymphangiogenesis

The vascular endothelial growth factors (VEGF-A, -C, -D) and the VEGF receptors (VEGFR-1, -2, -3) are important molecular markers in angiogenesis lymphangiogenesis. This study elucidates prognostic significance of these tumor cells as well stroma resected non-small cell lung cancer tumors.Tumor tissue samples from 335 patients with stage I to IIIA disease were obtained microarrays constructed duplicate cores surrounding stromal each specimen. Immunohistochemistry was used evaluate expression marker. Microvessel density assessed by CD34 immunohistochemical staining.In univariate analyses, high VEGF-A (P = 0.0005), VEGFR-1 0.013), VEGFR-2 0.006), VEGFR-3 0.0003) negative indicators for disease-specific survival (DSS). In stroma, however, 0.017), VEGF-C 0.003), VEGF-D 0.009), 0.01), 0.019) correlated good prognosis. There no significant correlation between microvessel DSS. multivariate 0.007) an independent factor DSS, whereas cells, 0.004) had positive impact.These first microarray data cancers showing a impact highly expressed angiogenic major indicator.