Data from Enhancing Chemosensitivity of Breast Cancer Stem Cells by Downregulating SOX2 and ABCG2 Using Wedelolactone-encapsulated Nanoparticles

• DOI: 10.1158/1535-7163.c.6538497.v1
• Publication Date: 2023-04-03T20:51:15Z
• Authors: Sreemanti Das; Pritha Mukherjee; Ranodeep Chatterjee; Zarqua Jamal; Urmi Chatterji
• Keywords: Abcg2; SKBR3

<div>Abstract<p>A major caveat in the treatment of breast cancer is disease recurrence after therapeutic regime at both local and distal sites. Tumor relapse attributed to persistence chemoresistant stem cells (CSC), which need be obliterated along with conventional chemotherapy. Wedelolactone, a naturally occurring coumestan, demonstrates anticancer effects different cells, although several limitations, mostly ineffective against CSCs. To enhance its biological activity additionally target CSCs, wedelolactone-encapsulated PLGA nanoparticles (nWdl) were formulated. Initial results indicated that nanoformulation wedelolactone not only increased uptake CSC population, it enhanced drug retention sustained release within cells. Enhanced was achieved by downregulation SOX2 ABCG2, contribute resistance In addition, nWdl prevented epithelial-to-mesenchymal transition, suppressed cell migration invasion, reduced percentage (BCSC) MDA-MB-231 When administered combination paclitaxel, known BCSCs, sensitized paclitaxel ALDH<sup>+</sup> BCSCs mammospheres. Furthermore, growth solid tumors mice also CD44<sup>+</sup>/CD24<sup>−/low</sup> population. Taken together, our data imply decreased metastatic potential chemosensitivity through coordinated regulation pluripotent efflux genes, thereby provides an insight into effective delivery specifically for obliterating BCSCs.</p></div>